Sample Processing SOP
Standard operating procedures for cancer biomarker testing
Important: Always follow safety protocols when handling biological samples. Ensure all PPE is worn throughout the procedure.
Cancer Biomarker Panel Processing
Version 2.1
This SOP describes the complete workflow for processing blood samples for cancer biomarker analysis including CEA, CA 19-9, AFP, PSA, CA 125, and HCG.
1
Sample Reception & Verification
Verify sample integrity and documentation upon receipt
• Check sample ID matches requisition
• Verify sample volume (minimum 250 μL)
• Inspect for hemolysis or clotting
• Record temperature and time of receipt
• Verify sample volume (minimum 250 μL)
• Inspect for hemolysis or clotting
• Record temperature and time of receipt
2
Sample Preparation
Prepare samples for automated analysis
• Centrifuge if needed (3000 RPM, 10 min)
• Aliquot samples into analysis tubes
• Label with barcode scanner
• Load into instrument rack
• Aliquot samples into analysis tubes
• Label with barcode scanner
• Load into instrument rack
3
Quality Control
Run daily QC before patient samples
• Run Level 1 and Level 2 controls
• Verify results within acceptable range
• Document QC results in log
• Proceed only if QC passes
• Verify results within acceptable range
• Document QC results in log
• Proceed only if QC passes
4
Analysis
Execute automated analysis protocol
• Load samples into analyzer
• Select appropriate test panels
• Monitor analysis progress
• Review results for flags or errors
• Select appropriate test panels
• Monitor analysis progress
• Review results for flags or errors
5
Result Review & Reporting
Validate and release results
• Review all results for clinical significance
• Flag abnormal values for pathologist review
• Enter results into LIMS system
• Generate and transmit reports
• Flag abnormal values for pathologist review
• Enter results into LIMS system
• Generate and transmit reports